Nick Fischer

Contact information

Lawrence Livermore National Laboratory
7000 East Avenue, L-452
Livermore, CA 94550
phone: 925 422 6144


Post-Doctoral Researcher, Lawrence Livermore National Laboratory, 2005-2008
Ph.D. in Cellular and Molecular Biology, University of Massachusetts – Amherst, 2005
B.S. in Biology, West Chester University of Pennsylvania, 1998


1.  Nanolipoprotein particles (NLPs) as an in vivo delivery platform for biomedical applications.
  Platform development:

  • Development of functionalization strategies for biomolecule incorporation
  • Quantitative characterization of NLP components
  • Protein expression and purification
  • Large-scale NLP production

  Platform applications:

  • Vaccine development
  • Agonist delivery for innate immune stimulation
  • Small-molecule targeted delivery

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2.  Strategies for streamlining the development and optimization of oligonucleotide aptamers

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Recent publications


Weilhammer D, Dunkle AD, Blanchette CD, Fischer NO, Corzett M, Lehmann D, Boone T, Hoeprich P, Driks A, Rasley A (2017) Enhancement of antigen-specific CD4+ and CD8+ T cell responses using a self-assembled biologic nanolipoprotein particle vaccine. Vaccine, 35, 1475-1481. PMID: 28214044.


Gilmore SF, Blanchette CD, Scharadin TM, Hura GL, Rasley A, Corzett M, Pan C-X, Fischer NO*, Henderson PT* (2016) Lipid crosslinking of nanolipoprotein particles substantially enhances serum stability and cellular uptake. ACS Applied Materials and Interfaces, 8, 20549-20557. (*Co-corresponding authors) PMID: 27411034.

Enright HE, Felix SH, Fischer NO, Mukerjee E, Soscia D, Mcnerney M, Kulp K, Zhang J, Page G, Miller P, Ghetti A, Wheeler EK, Pannu S (2016) Long-term non-invasive interrogation of human dorsal root ganglion neuronal cultures on an integrated microfluidic multielectrode array platform. Analyst, 141, 5346-5357. PMID: 27351032.


Laurence TA, Ly S, Bourguet F, Fischer NO, Coleman MA (2014) Fluorescence correlation spectroscopy at micromolar concentrations without optical nanoconfinement. Journal of Physical Chemistry B, 118, 9662-9667. PMID: 25060197.

Fischer NO, Weilhammer DR, Dunkle A, Thomas C, Hwang M, Corzett M, Lychak C, Mayer W, Urbin S, Collette N, Chang JC, Loots GG, Rasley A, and Blanchette CD (2014) Evaluation of nanolipoprotein particles (NLPs) as an in vivo delivery platform, PLoS One, 9, e93342. PMID: 24675794.


Ly S, Bourguet F, Fischer NO, Lau EY, Coleman MA, and Laurence TA (2014) Quantifying interactions of a membrane protein embedded in a lipid nanodisc using fluorescence correlation spectroscopy. Biophysical Journal, 106, L05-L08. PMID:24461026.


Weilhammer DR, Blanchette CD, Fischer NO, Alam S, Loots GG, Corzett M, Thomas C, Lychak C, Dunkle AD, Ruitenberg JJ, Ghanekar SA, Sant AJ, Rasley A. (2013The use of nanolipoprotein particles to enhance the immunostimulatory properties of innate immune agonists against lethal influenza challengeBiomaterials, 34, 10305-18.

Yoon I, Baker SE, Kim K, Fischer NO, Heineck D, Wang Y, Esener SC, Sirbuly DJ (2013) Nanofiber near-field light-matter interactions for enhanced detection of molecular level displacements and dynamics. Nano Letters, 13, 1440-1445. PMID: 23517010.

Fischer NO, Rasley A, Corzett M, Hwang MH, Hoeprich PD, Blanchette CD (2013) Colocalized delivery of adjuvant and antigen using nanolipoprotein particles enhances the immune response to recombinant antigens. Journal of the American Chemical Society, 135, 2044-2047. PMID: 23331082.


Blanchette C, Lacayo CI, Fischer NO, Hwang M, Thelen MP (2012) Enhanced cellulose degradation using cellulase-nanosphere complexes. PloS ONE, 7, e42116. PMID: 22870287.


Fischer NO, Tarasow TM (2011) Identification and optimization of DNA aptamer binding regions using DNA microarrays. Methods in Molecular Biology, 723, 57-66. PMID: 21370059.


Fischer NO, Blanchette CD, Segelke BW, Corzett M, Chromy BA, Kuhn EA, Bench G, Hoeprich PD (2010) Isolation, characterization, and stability of discretely-sized nanolipoprotein particles assembled with apolipophorin-III. PloS ONE, 5, e11643. PMID: 20657844

Blanchette CD, Fischer NO, Corzett M, Bench G, Hoeprich PD (2010) Kinetic analysis of His-tagged protein binding to nickel-chelating nanolipoprotein particles. Bioconjugate Chemistry, 21, 1321-1330. PMID: 20586461

Fischer NO, Infante E, Ishikawa T, Blanchette CD, Bourne N, Hoeprich PD, Mason PW (2010) Conjugation to nickel-chelating nanolipoprotein particles increases the potency and efficacy of subunit vaccines to prevent West Nile encephalitis. Bioconjugate Chemistry, 21, 1018-1022. (communication) PMID: 20509624


Blanchette CD, Segelke BW, Fischer  N, Corzett MH, Kuhn EA , Cappuccio  JA, Benner WH, Coleman MA, Chromy BA, Bench G, Hoeprich PD,  Sulchek TA (2009) Characterization and purification of polydisperse reconstituted lipoproteins and nanolipoprotein particles. International Journal of Molecular Sciences, 10, 2958-2971. PMID: 19742178

Baker SE, Hopkins RC, Blanchette CD, Walsworth VL, Sumbad R, Fischer NO, Kuhn EA, Coleman M, Chromy BA, Létant SE, Hoeprich PD, Adams MWW, Henderson PT (2009) Hydrogen production by a hyperthermophilic membrane-bound hydrogenase in water soluble nanolipoprotein particles. Journal of the American Chemical Society, 131, 7508-7509. PMID: 19449869

Fischer NO, Blanchette CD, Chromy BA, Kuhn EA, Segelke BW, Corzett M, Bench G, Mason PW, Hoeprich PD (2009) Immobilization of His-tagged proteins on nickel-chelating nanolipoprotein particles. Bioconjugate Chemistry, 20, 460-465. PMID: 19239247


Schopf E, Fischer NO, Chen Y, Tok JBH (2008) Sensitive and selective viral DNA detection assay via microbead-based rolling circle amplification. Bioorganic and Medicinal Chemistry Letters, 18, 5871-5874. PMID: 18694640

Fischer NO, Tok JBH, Tarasow TM (2008) Massively parallel interrogation of aptamer sequence, structure and function. PloS ONE, 3, e2720. PMID: 18628955

Tok JBH, Fischer NO (2008) Single microbead SELEX for efficient ssDNA aptamer generation against botulinum neurotoxin. Chemical Communications, 16, 1883-1885. PMID: 18401506

Sirbuly DJ, Fischer NO, Huang SCJ, Artyukhin AB, Tok JBH, Bakajin O, and Noy A. (2008) Biofunctional subwavelength optical waveguides for biodetection. ACS Nano, 2, 255-262. (Profiled in Analytical Currents, Analytical Chemistry, 80, 2276) PMID: 19206625

Fischer NO, Tarasow TM, Tok JBH. (2008) Protein detection via direct enzymatic amplification of short DNA aptamers. Analytical Biochemistry, 373, 121-128. PMID: 17980857


Fischer NO, Tarasow TM, Tok JBH (2007) Aptasensors for biosecurity applications. Current Opinions in Chemical Biology, 11, 316-328. PMID: 17548236

Fischer NO, Tarasow TM, Tok JBH (2007) Heightened sense for sensing: recent advances in pathogen immunoassay sensing platforms. The Analyst, 132, 187-191. (Cover Article) PMID: 17325749