Brent Segelke

Contact information

Lawrence Livermore National Laboratory
7000 East Avenue, L-452
Livermore, CA 94550
phone: 925 424 4752


Post-Doctoral Fellow, Lawrence Livermore National Laboratory, 1997-2000

Post-Doctoral Fellow, The Scripps Research Institute, La Jolla, 1995-1997

Ph.D., Biophysics, University of California, San Diego (1995)

B.S., Chemistry, University of California, Davis (1989)

Research Interests

My long range research interest is to understand the molecular mechanism of bacterial infection, with structural biology as a key tool for elucidating molecular function of individual proteins or complexes thought to be involved in the course of infection. 

Selected Publications

Practical applications of structural genomics technologies for mutagen research (2011) Zemla A, Segelke BW, Mutat Res. Jun 17;722(2):165-70.

Structure of Rv1848 (UreA), the Mycobacterium tuberculosis urease gamma subunit (2010) Habel JE, Bursey EH, Rho BS, Kim CY, Segelke BW, Rupp B, Park MS, Terwilliger TC, Hung LW. Acta Crystallogr F. 66:781-6.

Cell-free expression for nanolipoprotein particles: building a high-throughput membrane protein solubility platform (2009) Cappuccio JA, Hinz AK, Kuhn EA, Fletcher JE, Arroyo ES, Henderson PT, Blanchette CD, Walsworth VL, Corzett MH, Law RJ, Pesavento JB, Segelke BW, Sulchek TA, Chromy BA, Katzen F, Peterson T, Bench G, Kudlicki W, Hoeprich PD Jr, Coleman MA. Methods Mol Biol. 498:273-96.

Cell-free co-expression of functional membrane proteins and apolipoprotein, forming soluble nanolipoprotein particles (2008) Cappuccio JA, Blanchette CD, Sulchek TA, Arroyo ES, Kralj JM, Hinz AK, Kuhn EA, Chromy BA, Segelke BW, Rothschild KJ, Fletcher JE, Katzen F, Peterson TC, Kudlicki WA, Bench G, Hoeprich PD, Coleman MA. Mol Cell Proteomics. 7(11):2246-53.

High throughput crystallography of TB drug targets (2007) Murillo AC, Li HY, Alber T, Baker EN, Berger JM, Cherney LT, Cherney MM, Cho YS, Eisenberg D, Garen CR, Goulding CW, Hung LW, Ioerger TR, Jacobs WR, James MN, Kim C, Krieger I, Lott JS, Sankaranarayanan R, Segelke BW, Terwilliger TC, Wang F, Wang S, Sacchettini JC. Infect Disord Drug Targets. 7(2):127-39.

Macromolecular crystallization with microfluidic free-interface diffusion (2005) Segelke B. Expert Rev Proteomics. 2(2):165-72.

High-throughput, fluorescence-based screening for soluble protein expression (2004) Coleman MA, Lao VH, Segelke BW, Beernink PT. J Proteome Res. 3(5):1024-32.

Crystal structure of Clostridium botulinum neurotoxin protease in a product-bound state: Evidence for noncanonical zinc protease activity (2004) Segelke B, Knapp M, Kadkhodayan S, Balhorn R, Rupp B. Proc Natl Acad Sci USA. 101(18):6888-93.

Laboratory scale structural genomics (2004) Segelke BW, Schafer J, Coleman MA, Lekin TP, Toppani D, Skowronek KJ, Kantardjieff KA, Rupp B. J Struct Funct Genomics. 5(1-2):147-57.

Conformational flexibility in the apolipoprotein E amino-terminal domain structure determined from three new crystal forms: implications for lipid binding (2000) Segelke BW, Forstner M, Knapp M, Trakhanov SD, Parkin S, Newhouse YM, Bellamy HD, Weisgraber KH, Rupp B. Protein Sci. 9(5):886-97.

Structures of two novel crystal forms of Naja naja naja phospholipase A2 lacking Ca2+ reveal trimeric packing (1998) Segelke BW, Nguyen D, Chee R, Xuong NH, Dennis EA. J Mol Biol. 279(1):223-32.

Crystal structure of mouse CD1: An MHC-like fold with a large hydrophobic binding groove (1997) Zeng Z, Castaño AR, Segelke BW, Stura EA, Peterson PA, Wilson IA. Science. 277(5324):339-45.