Lawrence Livermore National Laboratory
Ph.D., Geobiology, California Institute of Technology (2007)
My research is at the interface of environmental microbiology and biogeochemistry. My group applies an interdisciplinary approach to gain a predictive understanding of the metabolic activities of microbes and microbial communities in natural and perturbed environments. Current research directions include:
- Investigating the mechanism of uranium biomineralization by Caulobacter crescentus. This project is funded by a DOE Early Career Award for studying uranium resistance and regulation by Caulobacter, in collaboration with Drs. L. Shapiro and H. McAdams at Stanford University and Dr. P. Hu at Lawrence Berkeley National Laboratory. More info.
- Investigating metabolic interactions in a bacterial co-culture containing Clostridium cellulolyticum and Rhodopseudomonas palustris for biohydrogen production when grown on cellulosic materials. In collaboration with Ali Navid, also in the BBS Group at LLNL, we take a systems biology approach combining wet lab experiments with computational modeling of metabolic flux analysis.
- Applying a basic research component to support the development of a large-scale laboratory process that couples bioleaching with biosorption/recovery of critical materials including some rare earth elements. This research is in collaboration with David Reed at Idaho National Laboratory.
Transposon Sequencing Strategy for Studying Microbial Metal Resistance. Insertion mutants that are able to survive in the presence of selected heavy metals were pooled to generate a saturated Tn5 mutant library. Subsequent parallel amplification of individual transposon junctions yield DNA fragments reading out of transposon elements into adjacent genomic DNA sequences. DNA fragments carrying terminal adapters compatible with the Illumina flow-cell were sequenced by standard paired-end sequencing.
Yung, M. C., Park, D. M., Overton, K. W., Blow, M. J., Hoover, C. A., Smit, J., Murray, S. R., Ricci, D. P., Christen, B., Bowman, G. R., & Jiao, Y. (2015) Transposon mutagenesis paired with deep sequencing of Caulobacter crescentus under uranium stress reveals genes essential for detoxification and stress tolerance. J. Bacteriol. 197, 3160-72.
Jiao Y., A. Navid, B.J. Stewart, J.B. McKinlay, M. Thelen and J. Pett-Ridge. “Syntrophic metabolism of a co-culture containing Clostridium cellulolyticum with Rhodopseudomonas palustris on cellulose”. International Journal of Hydrogen Energy, 37: 11719-726 (2012). Doi 10.1016/j.ijhydene.2012.05.100
Jiao, Y., F. Qian, Y. Li, G. Wang, C.W. Saltikov, J.A. Granick, “Deciphering the electron transport pathway for graphene oxide reduction by Shewanella oneidensis MR-1”, Journal of Bacteriology, 193(14): 3662-3665 (2011). doi: 10.1128/JB.00201-11
Jiao, Y., P. D'haeseleer, B.D. Dill, M. Shah, N.C. Verberkmoes, R.L. Hettich, J.F. Banfield, M.P. Thelen, “Identification of biofilm matrix-associated proteins from an acid mine drainage microbial community.” Applied and Environmental Microbiology, 77(15): 5230-5237 (2011). doi: 10.1128/AEM.03005-10
Wang, G., F. Qian, C.W. Saltikov, Y. Jiao, Y. Li, “Microbial reduction of graphene oxide by Shewanella”, Nano Research, pp. 1-8, (2011). doi: 10.1007/s12274-011-0112-2
Wheeler, K.E., A. Zemla, Y. Jiao, D.S. Goltsman, S.W. Singer, J.F. Banfield and M.P. Thelen “Functional insights from Computational Modeling of Orphan Proteins Expressed in a Microbial Community.” Journal of Proteomics & Bioinformatics, 3: 266-274 (2010). doi:10.4172/jpb.1000150
Jiao, Y., G.D. Cody, A.K. Harding, P. Wilmes, M. Schrenk, K.E. Wheeler, J.F. Banfield, M.P. Thelen, “Characterization of extracellular polymeric substances from acidophilic microbial biofilms.” Applied and Environmental Microbiology, 76: 2916-2922 (2010). doi: 10.1128/AEM.02289-09
Jiao, Y.*, L.R. Croal*, A. Kappler, D.K. Newman, “Phototrophic Fe(II) oxidation in an atmosphere of H2: implications for Archean banded iron formations”, *co-first authors. Geobiology, 7 (1): 21-4 (2009). doi: 10.1111/j.1472-4669.2008.00185.x
Jiao, Y., D.K. Newman “The pio operon is essential for phototrophic Fe(II) oxidation in Rhodopseudomonas palustris TIE-1”, Journal of Bacteriology, 189 (5): 1765-1773 (2006). doi: 10.1128/JB.00776-06
Croal, L.R., Y. Jiao, D.K. Newman, “The fox Operon from Rhodobacter Strain SW2 Promotes Phototrophic Fe(II) Oxidation in Rhodobacter capsulatus SB1003”, Journal of Bacteriology, 189 (5): 1774-1782 (2006). doi: 10.1128/JB.01395-06
Jiao, Y., A.K. Kappler, L.R. Croal, D.K. Newman, “Isolation and Characterization of a Genetically Tractable Photoautotrophic Fe(II)-Oxidizing Bacterium, Rhodopseudomonas palustris Strain TIE-1. Applied and Environmental Microbiology 71: 4487-4496 (2005). doi: 10.1128/AEM.71.8.4487-4496.2005