Lawrence Livermore National Laboratory
B.S. in Biology, Fu-Jen Catholic University, 1970
Work at LLNL
I participate in research studies in immunology, microbiology, and molecular biology. I conduct wet lab experiments and maintain cell cultures for studies. I work with research scientists and postdocs, and prepare data analysis tables and figures needed for scientific papers.
Experience (30+ years)
1. Monoclonal antibody production: hybridoma culture, antibody purification, test of antibody by ELISA and Western blotting.
2. Protein biochemistry: cell lysis, chromatographic protein purification, gel electrophoresis, protein expression, electrophoretic transfer for N-terminal sequencing.
3. Molecular biology: bacterial culturing, genomic and plasmid DNA extraction, PCR, molecular cloning.
4. Laboratory safety and maintenance, ordering lab supplies, maintaining cell-line collections, and managing lab inventory and waste.
5. NLP assembling.
C. I. Lacayo, M. S. Hwang, S.-Y. Ding, M. P. Thelen (2013) Lignin depletion enhances the digestibility of cellulose in cultured xylem cells. PLoS ONE 8(7), e68266.
N. O. Fischer, A. Rasley, M. Corzett, M. H. Hwang, P. D. Hoeprich, C. D. Blanchette (2013) Colocalized delivery of adjuvant and antigen using nanolipoprotein particles enhances the immune response to recombinant antigens. Journal of the American Chemical Society, 135, 2044-2047.
C. Blanchette, C. I. Lacayo, N. O. Fischer, M. Hwang, M. P. Thelen (2012) Enhanced cellulose degradation using cellulase-nanosphere complexes. PLoS One 7(8), e42116.
C. I. Lacayo, A. Malkin, H.-Y. Holman, S.-Y. Ding, M. Hwang, MP Thelen (2010) Imaging cell wall architecture in single Zinnia elegans tracheary elements.
Plant Physiol 154, 121-33.